منابع مشابه
Endocytosis in Chang Liver Cells
The addition of 0.08 M sucrose to a culture medium containing Chang-strain human liver cells causes intense cytoplasmic vacuolation. Electron microscopy of these cells grown inferritin, time-lapse cinematography, and radioautography reveal that the vacuoles arise by endocytosis and that the sucrose is taken into the cell and localized in the vacuoles. Tracer studies demonstrate that sucrose-(3)...
متن کاملEstablishment of a hepatocyte steatosis model using Chang liver cells.
The objective of this study was to explore the experimental conditions for hepatocellular steatosis models of Chang liver cells induced by oleic acid (OA). For that, Chang liver cells were induced by different concentrations of OA for different periods. The MTT assay was used to detect hepatic cell activity, the Oil Red O staining was used to observe intracellular lipid droplets accumulation, a...
متن کاملIn vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
The liver is the metabolic center of the mammalian body and serves as a filter for the blood. The basic architecture of the liver is illustrated in figure 1 in which more than 85% of the liver mass is composed of hepatocytes and the remaining 15% of the cellular mass is composed of Kupffer cells (KCs), stellate cells (HSCs), and sinusoidal endothelial cells (SECs). SECs form the blood vessel ...
متن کاملAbsence of a liver-specific membrane protein in a strain of chang cells.
A strain of Chang cells. derived from a human liver line, was examined for the presence of a liver-specific membrane protein (LSP). as described by Meyer zum Buschenfelde. Indirect immunofluorescence and antisera to human. rat, and rabbit LSP were used to demonstrate that LSP was present on isolated human, rat. and rabbit hepatocytes but not on the Chang cells. The results indicate that these C...
متن کاملProtective effect of moutan cortex extract on acetaminophen-induced cytotoxicity in human Chang liver cells.
The aim of this study was to investigate the effect of Moutan Cortex on acetaminophen (AAP)-induced toxicity in human Chang liver cells. Cells were incubated with AAP (0-30 mM) to evaluate the drug's ability to reduce cytoviability. For the cells treated with 10, 20 and 30 mM AAP, LDH leakage was 39.8%, 49.0% and 57.6%, respectively. Administration of Moutan Cortex reduced cytotoxicity in a dos...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.50.3.804